GPX4 antibody

References #1
Category:
Catalogue No.: 
FNab10620
Alias: 
Phospholipid hydroperoxide glutathione peroxidase GPX4 (PHGPx)|Glutathione peroxidase 4 (GPx-4 antibody, GSHPx-4)|GPX4 antibody
Gene Name: 
GPX4
UniProt ID: 
P36969
Size Price
100μg Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
GPX4 antibody
Catalogue No.
FNab10620
Gene Name
GPX4
UniProt ID
P36969
Alias
Phospholipid hydroperoxide glutathione peroxidase GPX4 (PHGPx)|Glutathione peroxidase 4 (GPx-4 antibody, GSHPx-4)|GPX4 antibody
Isotype
IgG2b
Clone ID
5C8
Form
liquid
Purification
Protein A+G purification
Immunogen
glutathione peroxidase 4 (phospholipid hydroperoxidase)
Recommended dilution
WB: 1:500-1:2000; IHC: 1:500-1:2000; IF: 1:100-1:500
Purity
≥95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.


Figures for this product are not available in this catalogue.

Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

Aradığınızı bulamadınız mı? Tüm SSS sayfasına bakın veya bize yazın.

Cited Publications (1)

Sodium aescinate induces renal toxicity by promoting Nrf2/GPX4-mediated ferroptosis IF: 5.1

Chemico-Biological Interactions · 2024-03-08

Related Products

Support

Product Comparison

Compare