TJP1 antibody

References #2
Category:
Catalogue No.: 
FNab09751
Alias: 
Tight junction protein ZO-1|Tight junction protein 1|Zona occludens protein 1|Zonula occludens protein 1|TJP1|ZO1 antibody
Gene Name: 
TJP1
UniProt ID: 
Q07157
Size Price
100μg Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
TJP1 antibody
Catalogue No.
FNab09751
Gene Name
TJP1
UniProt ID
Q07157
Alias
Tight junction protein ZO-1|Tight junction protein 1|Zona occludens protein 1|Zonula occludens protein 1|TJP1|ZO1 antibody
Isotype
IgG
Form
liquid
Purification
Immunogen affinity purified
Immunogen
tight junction protein 1(zona occludens 1)
Recommended dilution
WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Tight junction(or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium(PMID: 20066090). ZO-1(also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells(PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin(PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs(MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation(PMID: 22782886).


Figures for this product are not available in this catalogue.

Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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Cited Publications (2)

Astragaloside IV Alleviates Ulcerative Colitis Progression by Inhibiting WDR5‐Mediated ENO1 H3K4me3 Modification IF: 3.1

Kaohsiung Journal of Medical Sciences · 2025-10-17

Corilagin alleviates ferroptosis in diabetic retinopathy by activating the Nrf2 signaling pathway IF: 6.9

Biomedicine & Pharmacotherapy · 2024-09-13

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