Products
Porcine GHRL (Ghrelin) ELISA Kit
This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with GHRL. GHRL in the sample or standard competes with a fixed amount of biotin-labeled GHRL for sites on a pre-coated antibody specific to GHRL. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of GHRL in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
- Catalogue No.:
- EP0210
- Alias:
- Appetite-regulating hormone ELISA Kit , Growth hormone secretagogue ELISA Kit , Growth hormone-releasing peptide ELISA Kit , Motilin-related peptide ELISA Kit , GHRL ELISA Kit
- Gene Name:
- GHRL
- UniProt ID:
- Q58IV6
- Product Name
- Porcine GHRL (Ghrelin) ELISA Kit
- Catalogue No.
- EP0210
- Gene Name
- GHRL
- UniProt ID
- Q58IV6
- Alias
- Appetite-regulating hormone ELISA Kit , Growth hormone secretagogue ELISA Kit , Growth hormone-releasing peptide ELISA Kit , Motilin-related peptide ELISA Kit , GHRL ELISA Kit
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Competitive ELISA, Coated with Antigen
- Detection Wavelength
- OD450
- Reaction Duration
- 2 hours
- Range
- 3.12-200pg/ml
- Sensitivity
- 1.875pg/ml
- Specificity
- Specifically binds with GHRL , no obvious cross reaction with other analogues.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Kit Components
-
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C E003 Lyophilized Biotin-labeled Antibody(Concentrated) 1vial 1vial E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E024 TMB Substrate 5ml 10ml E005 Purified Water 200ul 200ul 2-8°C E039 Sample Dilution Buffer 10ml 20ml E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Typical Standard Curve
-
STD.(ng/ml) OD-1 OD-2 Average 0 1.991 2.093 2.032 3.125 1.723 1.811 1.758 6.25 1.32 1.387 1.347 12.5 0.978 1.028 0.998 25 0.726 0.763 0.741 50 0.572 0.602 0.584 100 0.361 0.379 0.368 200 0.223 0.235 0.228 - Precision
-
Item Sample n Mean (ng/ml) Standard deviation CV (%) Inter-assay Precision Sample 1 2 3 1 Inter-assay Precision n 20 20 20 20 Inter-assay Precision Mean (pg/ml) 5.82 23.18 96.94 5.94 Inter-assay Precision Standard deviation 0.37 1.49 5.76 0.25 Inter-assay Precision CV(%) 6.43 6.43 5.94 4.21
Figures for this product are not available in this catalogue.
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Dispatch Time
- About 3 working days
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EP0210 — Porcine GHRL (Ghrelin) ELISA Kit