Mouse RF-IgM(Rheumatoid Factor IgM) ELISA Kit

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This kit is based on indirect ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Biotinylated antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with RF-IgM bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of RF-IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
EM4271
Alias: 
Rheumatoid Factor IgM ELISA Kit
Gene Name: 
RF-IgM
MSDS
Size Price
48T/96T Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
Mouse RF-IgM(Rheumatoid Factor IgM) ELISA Kit
Catalogue No.
EM4271
Gene Name
RF-IgM
Alias
Rheumatoid Factor IgM ELISA Kit
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Indirect Elisa
Detection Wavelength
OD450
Reaction Duration
4 hours
Range
4.688-300IU/ml
Sensitivity
2.813IU/ml
Specificity
Specifically binds with RF-IgM , no obvious cross reaction with other analogues.
Storage
2-8°C(Sealed), Don't cryopreserve.
Kit Components
Kit ComponentsItemSize(48T)Size(96T) Storage Condition for Opened Kit
E001ELISA Microplate(Dismountable) 8×68×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002Lyophilized Standard 1vial2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E003Biotin-labeled Antibody(Concentrated, 100X) 60ul120ul 2-8°C (Avoid Direct Light)
E034HRP-Streptavidin Conjugate(SABC, 100X) 60ul120ul
E024TMB Substrate 5ml10ml
E039Sample Dilution Buffer 10ml20ml 2-8°C
E040Antibody Dilution Buffer 5ml10ml
E049SABC Dilution Buffer 5ml10ml
E026Stop Solution 5ml10ml
E038Wash Buffer(Concentrated, 25X) 15ml30ml
E006Plate Sealer 3 pieces5 pieces
E007Product Description 1 copy1 copy
Typical Standard Curve
STD.(ng/ml)OD-1OD-2Average
00.0940.0990.096
4.6880.120.1260.122
9.3750.170.1780.173
18.750.2280.240.233
37.50.3910.4110.399
750.7680.8080.784
1501.3591.4291.387
3002.1132.2212.156
Precision
ItemSamplenMean (ng/ml)Standard deviationCV (%)
Inter-assay Precision Sample1231
Inter-assay Precision n20202020
Inter-assay Precision Mean (IU/ml)9.435.94150.89.05
Inter-assay Precision Standard deviation0.491.636.920.41
Inter-assay Precision CV(%)5.234.544.594.5


Figures for this product are not available in this catalogue.

Storage
2-8°C(Sealed), Don't cryopreserve.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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Cited Publications (4)

The Spleen Modulates the Balance of Natural and Pathological Autoantibodies in a Mouse Model of Autoimmune Arthritis IF: 4.9

International Journal of Molecular Sciences · 2024-11-08

Splenectomy at Early stage of Autoimmune Arthritis Delayed Inflammatory Response and Reduced Joint Deterioration in Mice IF: 4.6

Clinical & Experimental Immunology · 2024-02-18

The immunological role of the spleen in rheumatoid arthritis

Pécsi Egyetemi Archívum · 2023-06-30

Splenectomy modulates the immune response but does not prevent joint inflammation in a mouse model of RA IF: 4.33

Clinical & Experimental Immunology · 2022-05-27

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