Mouse Kim-1(Kidney Injury Molecule 1) ELISA Kit

References #5
Category:

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Kim-1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Kim-1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Kim-1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
EM0126
Alias: 
Hepatitis A virus cellular receptor 1 ELISA Kit , HAVcr-1 ELISA Kit , Kidney injury molecule 1 ELISA Kit , KIM-1 ELISA Kit , T-cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit , TIMD-1 ELISA Kit , T-cell immunoglobulin mucin receptor 1 ELISA Kit , TIM, TIM-1 ELISA Kit , T-cell membrane protein 1 ELISA Kit , HAVCR1 ELISA Kit , KIM1 ELISA Kit , TIM1 ELISA Kit , TIMD1 ELISA Kit
Gene Name: 
Kim-1
UniProt ID: 
Q5QNS5
MSDS
Size Price
48T/96T Inquiry
Dispatch Time: About 3 working days
Contact Us
Product Name
Mouse Kim-1(Kidney Injury Molecule 1) ELISA Kit
Catalogue No.
EM0126
Gene Name
Kim-1
UniProt ID
Q5QNS5
Alias
Hepatitis A virus cellular receptor 1 ELISA Kit , HAVcr-1 ELISA Kit , Kidney injury molecule 1 ELISA Kit , KIM-1 ELISA Kit , T-cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit , TIMD-1 ELISA Kit , T-cell immunoglobulin mucin receptor 1 ELISA Kit , TIM, TIM-1 ELISA Kit , T-cell membrane protein 1 ELISA Kit , HAVCR1 ELISA Kit , KIM1 ELISA Kit , TIM1 ELISA Kit , TIMD1 ELISA Kit
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
4 hours
Range
7.813-500pg/ml
Sensitivity
4.688pg/ml
Specificity
Specifically binds with Kim-1 , no obvious cross reaction with other analogues.
Storage
2-8°C(Sealed), Don't cryopreserve.
Kit Components
Kit ComponentsItemSize(48T)Size(96T) Storage Condition for Opened Kit
E001ELISA Microplate(Dismountable) 8×68×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002Lyophilized Standard 1vial2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E003Biotin-labeled Antibody(Concentrated, 100X) 60ul120ul 2-8°C (Avoid Direct Light)
E034HRP-Streptavidin Conjugate(SABC, 100X) 60ul120ul
E024TMB Substrate 5ml10ml
E039Sample Dilution Buffer 10ml20ml 2-8°C
E040Antibody Dilution Buffer 5ml10ml
E049SABC Dilution Buffer 5ml10ml
E026Stop Solution 5ml10ml
E038Wash Buffer(Concentrated, 25X) 15ml30ml
E006Plate Sealer 3 pieces5 pieces
E007Product Description 1 copy1 copy
Typical Standard Curve
STD.(ng/ml)OD-1OD-2Average
00.0640.0660.065
7.8120.1190.1230.121
15.6250.2270.2330.23
31.250.3960.4080.402
62.50.6780.6980.688
1250.9310.9590.945
2501.3531.3931.373
5002.072.132.1
Precision
ItemSamplenMean (ng/ml)Standard deviationCV (%)
Inter-assay Precision Sample1231
Inter-assay Precision n20202020
Inter-assay Precision Mean (pg/ml)16.0162.66250.915.74
Inter-assay Precision Standard deviation0.782.9313.220.84
Inter-assay Precision CV(%)4.884.685.275.32


Figures for this product are not available in this catalogue.

Storage
2-8°C(Sealed), Don't cryopreserve.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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Cited Publications (5)

Pathology-Responsive Nanoprobes for NIR-II Imaging of Acute Kidney Injury IF: 6.7

Analytical Chemistry · 2026-05-15

Rab27a Deficiency Mitigates Mitochondrial Damage by Upregulating PPAR‐γ in Sepsis Associated Acute Kidney Injury IF: 4.2

The FASEB Journal · 2026-03-06

Lithospermic acid regulates macrophage polarization via AMPKα1 activation and ameliorates sepsis associated acute kidney injury in mice IF: 4.7

International Immunopharmacology · 2025-11-14

A Quinoxaline Derivative as a New Therapeutic Agent for Sepsis through Suppression of TLR4 Signaling Pathways IF: 4.5

Inflammation · 2025-05-09

Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway IF: 3.3

Journal of Natural Medicines · 2023-09-15

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