Human Anti-Semaglutide Neutralizing antibody ELISA Kit

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This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti Semaglutide Neutralizing antibody antibody. Sample or standard completely mixes with fixed amount of biotin-labeled Semaglutide Neutralizing antibody working solution and also add into each well. Semaglutide Neutralizing antibody in the sample or standard competes with biotin-labeled Semaglutide Neutralizing antibody to bind with antibody precoated on the microplate. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Semaglutide Neutralizing antibody in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

Catalogue No.: 
EH5241
Alias: 
Semaglutide Neutralizing antibody ELISA Kit
Gene Name: 
Semaglutide
UniProt ID: 
CAS: 910463-68-2
MSDS
Size Price
96T Inquiry
Dispatch Time: About 3 working days
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Product Name
Human Anti-Semaglutide Neutralizing antibody ELISA Kit
Catalogue No.
EH5241
Gene Name
Semaglutide
UniProt ID
CAS: 910463-68-2
Alias
Semaglutide Neutralizing antibody ELISA Kit
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Competitive ELISA, Coated with Antibody
Detection Wavelength
OD450
Reaction Duration
2 hours
Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Specificity
Specifically binds with Semaglutide Neutralizing antibody , no obvious cross reaction with other analogues.
Storage
2-8°C(Sealed), Don't cryopreserve.
Typical Standard Curve
STD.(ng/ml)OD-1OD-2Average
02.082.142.11
0.7811.5131.5571.535
1.5631.2781.3161.297
3.1250.8840.910.897
6.250.7230.7430.733
12.50.4260.4380.432
250.270.2780.274
500.1970.2030.2
Precision
ItemSamplenMean (ng/ml)Standard deviationCV (%)
Inter-assay Precision Sample1231
Inter-assay Precision n20202020
Inter-assay Precision Mean (ng/ml)1.556.6924.991.54
Inter-assay Precision Standard deviation0.090.311.120.08
Inter-assay Precision CV(%)5.874.694.55.5


Figures for this product are not available in this catalogue.

Storage
2-8°C(Sealed), Don't cryopreserve.
Dispatch Time
About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.

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