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Human Asprosin ELISA Kit

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ELISA Kits

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Asprosin antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Asprosin bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Asprosin in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.:: EH4176
Alias:: Asprosin ELISA Kit , Fibrillin-1 ELISA Kit , FBN1 ELISA Kit , FBN1 ELISA Kit
Gene Name:: Asprosin
UniProt ID:: P35555

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Size	Price
48T/96T	Inquiry

Dispatch Time: About 3 working days

Product Name

Human Asprosin ELISA Kit

Catalogue No.

EH4176

Gene Name

Asprosin

UniProt ID

P35555

Alias

Asprosin ELISA Kit , Fibrillin-1 ELISA Kit , FBN1 ELISA Kit , FBN1 ELISA Kit

Sample Type

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Method

Sandwich ELISA, Double Antibody

Detection Wavelength

OD450

Reaction Duration

4 hours

Range

1.563-100ng/ml

Sensitivity

0.938ng/ml

Specificity

Specifically binds with Asprosin , no obvious cross reaction with other analogues.

Storage

2-8°C(Sealed), Don't cryopreserve.

Kit Components

Kit Components	Item	Size(48T)	Size(96T)	Storage Condition for Opened Kit
E001	ELISA Microplate(Dismountable)	8×6	8×12	Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E002	Lyophilized Standard	1vial	2vial	Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
E003	Biotin-labeled Antibody(Concentrated, 100X)	60ul	120ul	2-8°C (Avoid Direct Light)
E034	HRP-Streptavidin Conjugate(SABC, 100X)	60ul	120ul
E024	TMB Substrate	5ml	10ml
E039	Sample Dilution Buffer	10ml	20ml	2-8°C
E040	Antibody Dilution Buffer	5ml	10ml
E049	SABC Dilution Buffer	5ml	10ml
E026	Stop Solution	5ml	10ml
E038	Wash Buffer(Concentrated, 25X)	15ml	30ml
E006	Plate Sealer	3 pieces	5 pieces
E007	Product Description	1 copy	1 copy

Typical Standard Curve

STD.(ng/ml)	OD-1	OD-2	Average
0	0.104	0.108	0.106
1.562	0.195	0.201	0.198
3.125	0.237	0.243	0.24
6.25	0.336	0.346	0.341
12.5	0.512	0.526	0.519
25	0.889	0.915	0.902
50	1.327	1.365	1.346
100	2.043	2.103	2.073

Precision

Item	Sample	n	Mean (ng/ml)	Standard deviation	CV (%)
Inter-assay Precision	Sample	1	2	3	1
Inter-assay Precision	n	20	20	20	20
Inter-assay Precision	Mean (ng/ml)	2.99	12.47	48.45	3.22
Inter-assay Precision	Standard deviation	0.14	0.61	2.34	0.15
Inter-assay Precision	CV(%)	4.69	4.89	4.82	4.68

Figures for this product are not available in this catalogue.

Storage: 2-8°C(Sealed), Don't cryopreserve.
Dispatch Time: About 3 working days

Ordering & Delivery

How do I place an order?

Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.

What is the dispatch time?

In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.

Do you ship outside Türkiye?

Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.

Storage & Handling

How should an unopened kit be stored?

At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.

How long is an opened kit stable?

It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.

What should I do if the shipment arrives warm?

Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.

Assay Questions

Which sample types can I use?

The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.

My background is too high. What should I check?

The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.

Can I run fewer wells than the kit provides?

Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.

Technical Support

Something did not work. What do you need from me?

Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.

Do you provide a certificate of analysis?

Yes, per lot. Ask for it with the catalogue number and lot number.

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Cited Publications (11)

Study of the Correlation Between Asprosin and Insulin Resistance in Obese and Normal-Weight PCOS Patients

Baghdad Science Journal · 2026-07-24

Association of Salivary Asprosin and RBP4 Levels in Periodontitis Patients with Varying Waist-to-Height Ratios: A Case–Control Study IF: 2.1

European Journal of Dentistry · 2026-04-03

Renal and metabolic effects of semaglutide plus canagliflozin vs canagliflozin alone in type 2 diabetic nephropathy IF: 4.6

World Journal of Diabetes · 2026-02-27

Elevated asprosin expression exacerbates synovial inflammation by PPAR-γ-dependent mechanisms in rheumatoid arthritis IF: 3.8

Clinical Immunology · 2026-02-13

Association between asprosin and metabolic syndrome in people living with human immunodeficiency virus: A case–control study IF: 1.4

Medicine · 2025-10-24

Isthmin 1, matrix metalloproteinase 8 and asprosin as potential biomarkers in periodontitis with obesity IF: 3.1

BMC Oral Health · 2025-10-17

Comparative Analysis of Afamin, Asprosin, and Pentraxin 3 Levels in Women with PCOS and Women with Unexplained Infertility IF: 2.1

Medical Science Monitor · 2025-10-17

Post-ERCP Pancreatitis and Serum Asprosin: A Potential Marker Associated with Beta Cell Damage

Journal of Ankara University Faculty of Medicine · 2025-10-17

Asprosin‐FABP5 Interaction Modulates Mitochondrial Fatty Acid Oxidation through PPARα Contributing to MASLD Development IF: 14.3

Advanced Science · 2025-04-25

Circulating asprosin concentrations in individuals with new-onset type 2 diabetes and prediabetes IF: 5.1

Diabetes Research and Clinical Practice · 2024-06-14

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